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This protein carries a human IgG1 Fc tag at the C-terminus
The protein has a calculated MW of 72.6 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human cluster Ⅲ Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human cluster Ⅲ Protein, Fc Tag (Cat. No. LR1-H5255) is more than 90% and the molecular weight of this protein is around 185-215 kDa verified by SEC-MALS.
Loaded Unconjugated Human PDGF-BB, His,Avitag (Cat. No. PDB-H5127) on NTA Biosensor, can bind Human cluster Ⅲ Protein, Fc Tag (Cat. No. LR1-H5255) with an affinity constant of 20 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Loaded Human cluster Ⅲ Protein, Fc Tag (Cat. No. LR1-H5255) on Protein A Biosensor, can bind Human APOE3, His Tag (Cat. No. APE-H5246) with an affinity constant of 8.7 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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