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T7 RNA Polymerase is a DNA-dependent RNA polymerase with strict specificity for the T7 phage promoter. The enzyme is widely used for the synthesis of specific transcripts from DNA in the 5'→ 3' direction, as well as being a suitable model for studying the mechanisms of transcription. The RNA produced by T7 RNA Polymerase is suitable for many downstream applications.
Synthesis of the single-strand RNA
Synthesis of highly labeled RNA probes
Synthesis of precursors of siRNA
One unit is defined as the amount of enzyme that will incorporate 1 nmol ATP into acid-insoluble material in a total reaction volume of 50 μl in 1 hour at 37°C.
>95% as determined by SDS-PAGE.
Supplied as 0.2 μm filtered solution in 50 mM Tris,100 M NaCl,pH8 with glycerol as protectant.
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This product is supplied and shipped on dry ice.