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Your Position: Home > Protein > GPRC5D > GPD-HF2D7

FITC-Labeled Human GPRC5D Protein, Flag,His Tag (Nanodisc)

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  • Synonym
    GPRC5D
  • Source
    FITC-Labeled Human GPRC5D Protein, Flag,His Tag(GPD-HF2D7) is expressed from human 293 cells (HEK293). It contains AA Tyr 2 - Phe 192 & Glu 197 - Leu 262 (Accession # Q9NZD1-2).
    Predicted N-terminus: Asp
  • Molecular Characterization
    GPRC5D Structure

    This protein carries flag tag at the N-terminus and polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 51.8 kDa. The protein migrates as 19-27 kDa, 36-37 kDa and 66 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

    Nanodiscs are a new class of model membranes that are being used to solubilize and study a range of integral membrane proteins and membrane-associated proteins. The Nanodisc bilayer is bounded by a membrane scaffold protein (MSP1D1) coat that confers enhanced stability and a narrow particle size distribution.

    The nanodisc assembles from a mixture of full length membrane protein in detergent, phospholipid micelles and membrane scaffold protein(MSP1D1) upon removal of the detergent.

  • Conjugate

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >80% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
*The isotype control of empty/mock nanodisc (Cat. No. APO-H51H3) is sold separately and not included in protein, you can follow this link for product information.
SDS-PAGE
GPRC5D SDS-PAGE

FITC-Labeled Human GPRC5D Protein, Flag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 80% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-ELISA
 GPRC5D ELISA

Immobilized FITC-Labeled Human GPRC5D Protein, Flag,His Tag (Cat. No. GPD-HF2D7) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human GPRC5D antibody, Human IgG4 with a linear range of 0.2-16 ng/mL (QC tested).

Bioactivity-SPR
 GPRC5D SPR

Anti-GPRC5D Antibody, Human IgG4 captured on Protein A Chip can bind FITC-Labeled Human GPRC5D Protein, Flag,His Tag (Cat. No. GPD-HF2D7) with an affinity constant of 9.29 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    The protein encoded by this gene is a member of the G protein-coupled receptor family; however, the specific function of this gene has not yet been determined. (provided by RefSeq, Jul 2008)
  • Clinical and Translational Updates

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