This protein carries a flag tag at the N-terminus, followed by a polyhistidine tag, and a GFP tag at the C-terminus
The protein has a calculated MW of 54.0 kDa. The protein migrates as 22 kDa, 25 kDa, 51-52 kDa and 100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Nanodiscs are a new class of model membranes that are being used to solubilize and study a range of integral membrane proteins and membrane-associated proteins. The Nanodisc bilayer is bounded by a membrane scaffold protein (MSP1D1) coat that confers enhanced stability and a narrow particle size distribution.
The nanodisc assembles from a mixture of full length membrane protein in detergent, phospholipid micelles and membrane scaffold protein(MSP1D1) upon removal of the detergent.
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 530 nm
>80% as determined by SDS-PAGE.
Supplied as 0.2 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Fluorescent Human Claudin-6 Protein, Flag,His,GFP Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 80%.
Immobilized Fluorescent Human Claudin-6 Protein, Flag,His,GFP Tag (Cat. No. CL6-HF2M9) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human Claudin 6 antibody, Human IgG1 with a linear range of 0.1-3 ng/mL (QC tested).
Anti-Claudin-6 Antibody, Human IgG1 captured on Protein A Chip can bind Fluorescent Human Claudin-6 Protein, Flag,His,GFP Tag (Cat. No. CL6-HF2M9) with an affinity constant of 34.1 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
2e5 of anti-Claudin-6 CAR-293 cells were stained with 100 μL of 1 μg/mL of Fluorescent Human Claudin-6 Protein, Flag,His,GFP Tag (Cat. No.CL6-HF2M9) and negative control protein respectively (Fig. A and B), FITC signal was used to evaluate the binding activity (QC tested).
5e5 of PBMCs were stained with Fluorescent Human Claudin-6 Protein, Flag,His,GFP Tag (Cat. No. CL6-HF2M9) and anti-CD3 antibody, washed and then analyzed with FACS. PE signal was used to evaluate the expression of CD3+ T cells in PBMCs, and FITC signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $1900.00
Price(USD) : $5700.00
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