This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 92.3 kDa. The protein migrates as 100 kDa and 120 kDa (Cadherin), and 48 kDa (Propeptide) under reducing (R) condition (SDS-PAGE) due to different glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human E-Cadherin, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Authors: Campbell HK, et al
Journal: J Cell Biol 2019
Application: Magnetic bead force assays & 3D atomic force microscopy
Authors: Eshghi A, et al.
Journal: Cell Microbiol 2018
Authors: Kevin Suffoletto, et al.
Journal: J Biomech 2018
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