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Your Position: Home > Bio-Markers & CD Antigens > Growth Hormone R > Human GHR /GHBP /Growth Hormone Receptor Protein, With C-Fc Tag

Human Growth Hormone R (GHR) Protein, Fc Tag

  • Synonym
    GHR,GHBP,GH receptor
  • Source
    Human Growth Hormone R, Fc Tag (GHR-H5257) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Tyr 264 (Accession # P10912).
    Predicted N-terminus: Ala 27
  • Molecular Characterization
    GHR(Ala 27 - Tyr 264) P10912

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 54.3 kDa. As a result of glycosylation, the protein migrates as 66-85 kDa under reducing (R) condition, and 135-160 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Growth Hormone R, Fc Tag (Cat. No. GHR-H5257) SDS-PAGE gel

Human Growth Hormone R, Fc Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Human Growth Hormone R, Fc TagHuman Growth Hormone R, Fc Tag (Cat. No. GHR-H5257) ELISA bioactivity

Immobilized Human GH, Tag Free (Cat. No. GH1-H4117) at 2μg/mL (100 μL/well) can bind Human GHR, Fc Tag (Cat. No. GHR-H5257) with a linear range of 0.19-3.1 ng/mL (QC tested).

  • Background
    Growth hormone receptor (GHR) is also known as somatotropin receptor, growth hormone-binding protein (GHBR), which belongs to the type I cytokine receptor family or Type 1 subfamily. GHR contains one fibronectin type-III domain. GHR / GHBR is expressed in various tissues with high expression in liver and skeletal muscle. The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. GHR is receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway. The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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