>Immune Checkpoint Proteins
>Cynomolgus PDCD1 /PD1 Protein, With C-Fc Tag
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 42.6 kDa. As a result of different glycosylation, the protein migrates as 55-66 kDa under reducing (R) condition, and 110-140 kDa under non-reducing (NR) condition (SDS-PAGE).
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Cynomolgus PD-1, Fc Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Authors: Yam Alice, et al.
Journal: US20180142022A1 2018
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