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Your Position: Home > Immune Checkpoint Proteins > PD-1 > Cynomolgus PDCD1 /PD1 Protein, With C-Fc Tag

Cynomolgus PD-1 / PDCD1 Protein, Fc Tag

  • Synonym
    PDCD1,PD1,CD279,SLEB2
  • Source
    Cynomolgus PD-1, Fc Tag (PD1-C5254) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # B0LAJ3).
    Predicted N-terminus: Leu 25
  • Molecular Characterization
    Online(Leu 25 - Gln 167) B0LAJ3

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 42.6 kDa. The protein migrates as 53-60 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Cynomolgus PD-1, Fc Tag (Cat. No. PD1-C5254) SDS-PAGE gel

Cynomolgus PD-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Cynomolgus PD-1, Fc TagCynomolgus PD-1, Fc Tag (Cat. No. PD1-C5254) ELISA bioactivity

Immobilized Human PD-L1, Mouse Fc Tag (Cat. No. PD1-H52A3) at 5 μg/mL (100 μL/well) can bind Cynomolgus PD-1, Fc Tag (Cat. No. PD1-C5254) with a linear range of 3-33 ng/mL (QC tested).

Cynomolgus PD-1, Fc TagCynomolgus PD-1, Fc Tag (Cat. No. PD1-C5254) ELISA bioactivity

Immobilized Cynomolgus PD-1, Fc Tag (Cat. No. PD1-C5254) at 2 μg/mL (100 μL/well) can bind Nivolumab with a linear range of 0.1-3 ng/mL (Routinely tested).

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