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Your Position: Home > Kits > Mesothelin > CEA-B030

ClinMax™ Human Soluble Mesothelin ELISA Kit, PRO

For research use only.
Product Details
Assay TypeSandwich-ELISA
AnalyteSoluble Mesothelin
Format96T
ReactivityHuman
Regulatory StatusPRO
Sensitivity70.0 pg/mL
Standard Curve Range156.25 pg/mL-10000 pg/mL
Assay Time2 hr 45 min
Suitable Sample TypeFor the quantitative determination of human Soluble Mesothelin in Cell Culture Supernatants, Plasma, Serum.
Sample volume50 μL
Materials Provided
IDComponentsSize
CEA030-B01Pre-coated Anti-Soluble Mesothelin Antibody Microplate1 plate
CEA030-B02Human Soluble Mesothelin Standard20 μg ×2
CEA030-B03Biotin-Anti-Soluble Mesothelin Antibody Con. Solution100 μL
CEA030-B04Biotin-Antibody Dilution Buffer8 mL
CEA030-B05Streptavidin-HRP Con. Solution500 μL
CEA030-B06Streptavidin-HRP Dilution Buffer15 mL
CEA030-B0720× Washing Buffer50 mL
CEA030-B082×Sample Dilution Buffer15 mL ×2
CEA030-B09Substrate Solution12 mL
CEA030-B10Stop Solution6 mL
  • Background
    Human Soluble Mesothelin ELISA Kit is designed to measure human soluble Mesothelin protein in cell culture supernates, serum, and plasma. This assay kit is a sandwich-ELISA format. It contains recombinant human Mesothelin and a pair of antibodies against the recombinant factor, which are provided by ACROBiosystems. Results are obtained by four parameter logistic curve that were parallel to the standard curves obtained. The verification results indicate that this kit can be used for the quantitative determination of natural and recombinant human Mesothelin concentrations. The specificity has been verified.
  • Application

    The kit is developed for quantitative detection of human soluble Mesothelin in serum, plasma and cell culture supernates.

    It is for research use only.

  • Storage
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
  • Assay Principles
     Mesothelin Assay Principles
Typical Data Please refer to DS document for The assay protocol.
 Mesothelin TYPICAL DATA

For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of Soluble Mesothelin is less than 70.0 pg/mL.

  • Background: Mesothelin
    Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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