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Your Position: Home > Protein > HLA-A*0201 & B2M & p53 (HMTEVVRHC) > HLP-HP2H6

PE-Labeled Human HLA-A*02:01&B2M&p53 (HMTEVVRHC) Tetramer Protein

Streptavidin Protein - PE (STN-NP117, STN-NP119), supporting the assembly of your own MHC Tetramers!

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  • Synonym
    HLA-A*0201 & B2M & p53 (HMTEVVRHC)
  • Source
    PE-Labeled Human HLA-A*02:01&B2M&p53 (HMTEVVRHC) Tetramer Protein(HLP-HP2H6) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & HMTEVVRHC peptide (Accession # AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) & HMTEVVRHC).
    Predicted N-terminus: Gly 25 & Ile 21
  • Molecular Characterization

    PE-Labeled Human HLA-A*02:01&B2M&p53 (HMTEVVRHC) Tetramer Protein is assembled by biotinylated monomer and PE-labeled streptavidin.

    Biotinylated Human HLA-A*02:01&B2M&p53 (HMTEVVRHC) Complex Protein is produced by co-expression of HLA and B2M loaded with p53 peptide. Biotinylated Human HLA-A*02:01&B2M&p53 (HMTEVVRHC) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

  • Conjugate

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 1% BSA, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Bioactivity-ELISA
 HLA-A*0201 & B2M & p53 (HMTEVVRHC) ELISA

Immobilized PE-Labeled Human HLA-A*02:01&B2M&p53 (HMTEVVRHC) Tetramer Protein (Cat. No. HLP-HP2H6) at 1 μg/mL (100 μL/well) can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-2 ng/mL (QC tested).

 HLA-A*0201 & B2M & p53 (HMTEVVRHC) ELISA

Immobilized PE-Labeled Human HLA-A*02:01&B2M&p53 (HMTEVVRHC) Tetramer Protein (Cat. No. HLP-HP2H6) at 1 μg/mL (100 μL/well) can bind Anti-HLA-A2/p53R175H Antibody with a linear range of 0.004-0.125 μg/mL (Routinely tested).

  • Background
    TP53 has been recognized as a tumor suppressor. 50% of cancers carry a TP53 mutation while many others affect other pathway components. High-copy numbers of WT p53 peptide-MHC class I complexes were detected on tumor cells as compared to low copies on normal cells. The Human HLA-A*0201 p53 (HMTEVVRHC) complex protein is a complex of HLA-A*0201 of the MHC Class I, B2M, and HMTEVVRHC peptide of the p53.
  • Clinical and Translational Updates

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Price(USD) : $396.00

Price(USD) : $2280.00

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