This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 17.0 kDa. The protein migrates as 25-45 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Mouse PD-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Loaded Mouse PD-L1, Fc Tag (Cat. No. PD1-M5251) on Protein A Biosensor, can bind Mouse PD-1, His Tag (Cat. No. PD1-M5228) with an affinity constant of 6.9 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Authors: Galstyan A, et al
Journal: Nat Commun 43705
Authors: Ordikhani F, et al.
Journal: Jci Insight 43391
Authors: Li Y, et al.
Journal: Oncoimmunology 2015
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