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Your Position: Home > Protein > FGF-5 > FG5-H51H3

Human FGF-5 Protein, His Tag (SPR verified)

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  • Synonym
    FGF-5,Fibroblast growth factor 5,HBGF-5
  • Source
    Human FGF-5 Protein, His Tag(FG5-H51H3) is expressed from E. coli cells. It contains AA Glu 23 - Gly 268 (Accession # P12034-1).
    Predicted N-terminus: Glu 23
  • Molecular Characterization
    FGF-5 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 29.4 kDa. The protein migrates as 35-38 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
FGF-5 SDS-PAGE

Human FGF-5 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-SPR
 FGF-5 SPR

Human FGF-5 Protein, His Tag (Cat. No. FG5-H51H3) captured on CM5 chip via anti-His antibody can bind Human FGF R2 (IIIb), Fc Tag (Cat. No. FGB-H5256) with an affinity constant of 63.3 nM as determined in a SPR assay (Biacore 8K) (QC tested).

  • Background
    Fibroblast growth factor-5 (FGF5) is a member of the fibroblast growth factor (FGF) family and acts as a crucial regulator of hair growth and an oncogenic factor in several human cancers. Generally, Fibroblast growth factor 5 (FGF5) is widely expressed in embryonic but scarcely in adult tissues. Overexpression of FGF5 has been associated with prostate cancer, pancreatic cancer, breast cancer, renal cell carcinoma, etc. it was confirmed that FGF5 contributes to the malignant progression of human astrocytic brain tumors by both autocrine and paracrine effects.
  • Clinical and Translational Updates

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