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Product Details
Synonyms
FGF-17, Fibroblast growth factor 17, FGF17
Source
Human FGF-17 Protein, His Tag (FG7-H5144) is expressed from E. coli cells. It contains AA Thr 23 - Thr 216 (Accession # O60258-1).
Predicted N-terminus: Met
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 24.5 kDa. The protein migrates as 28-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE).
Endotoxin
Less than 0.1 EU per μg by the LAL method / rFC method.
Host Cell Protein
<0.5 ng/µg of protein tested by ELISA.
Host Cell DNA
<0.02 ng/μg of protein tested by qPCR.
Sterility
Negative
Mycoplasma
Negative
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Human FGF-17 Protein, His Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC
The purity of Human FGF-17 Protein, His Tag (Cat. No. FG7-H5144) was greater than 90% as determined by SEC-HPLC.
Bioactivity-ELISA
Immobilized Human FGF-17 Protein, His Tag (Cat. No. FG7-H5144) at 5 μg/mL (100 μL/well) can bind Human FGF R3 (IIIc), Fc Tag (Cat. No. FGC-H5256) with a linear range of 2-40 ng/mL (QC tested).
Protocol
Immobilized Human FGF-17 Protein, His Tag (Cat. No. FG7-H5144) at 5 μg/mL (100 μL/well) can bind Human FGF R4, Fc Tag (Cat. No. FG4-H5253) with a linear range of 0.078-0.625 μg/mL (QC tested).
Protocol
Bioactivity-CELL BASE
Human FGF-17 Protein, His Tag (Cat. No. FG7-H5144) stimulates proliferation of NIH-3T3 cells. The EC50 for this effect is 1.495-1.639 µg/mL (Routinely tested).
Protocol
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