DNase I (5U/μl)

产品描述（Product Details）

Deoxyribonuclease I (DNase I) is a single, glycosylated polypeptide that degrades unwanted single- and double-stranded DNA. The enzyme works by cleaving DNA into 5' phosphodinucleotide and small oligonucleotide fragments. DNase I is commonly added to cell lysis reagents to remove the viscosity caused by the DNA content in bacterial cell lysates or to remove the DNA templates from RNAs produced by in vitro transcription.

应用说明（Application）

1. Removal of contaminating genomic DNA from RNA samples
2. Degradation of DNA templates in transcription reactions
3. DNase I footprinting
4. Nick Translation

单位定义（Unit Definition）

One unit is defined as the amount of enzyme which will completely degrade 1 µg of DNA in 10 minutes at 37°C in 10 mM Tris·HCl (pH7.6), 2.5 mM MgCl2, 0.5 mM CaCl2.

质量控制（Quility Control）

RNase-free

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Supplied as 0.2 μm filtered solution in 20 mM Tris, pH7.6 with glycerol as protectant.

Contact us for customized product form or formulation.

运输（Shipping）

This product is supplied and shipped with blue ice, please inquire the shipping cost.

存储（Storage）

This product is stable after storage at:

1. This product is stable for up to 12 months at -20℃ from date of receipt.