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Your Position: Home > Protein > Cap-2’-O-Methyltransferase > CAE-V51H3

mRNA Cap 2'-O-Methyltransferase (50U/ul)

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  • Product Details
    mRNA CAP2-xymethyltransferase is a recombinant protein derived from vaccinia virus. The enzyme adds a methyl group at the 2´-O position of the first nucleotide adjacent to the cap structure at the 5´ end of the RNA. The enzyme utilizes S-adenosylmethionine (SAM) as a methyl donor to methylate capped RNA (cap-0) resulting in a cap-1 structure.mRNA Cap 2´-O-Methyltransferase specifically requires RNA with an m7GpppN cap as substrate.
  • Application
    1. mRNA cap-1 structure may improve expression during microinjection and transfection experiments.
  • Unit Definition
    One unit is defined as the amount of enzyme required to methylate 10 pmoles of 80 nt long capped RNA transcript in 1 hour at 37°C.
  • Quility Control
    RNase-free.
    DNase-free.
    Host Cell DNA : <0.02 ng/μg of protein tested by qPCR.
  • Formulation

    Supplied as 0.2 μm filtered solution in 20 mM Tris, 100 mM NaCl, 1 mM TCEP, 0.1 mM EDTA, pH 8.0 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    This product is stable after storage at:

    1. The product MUST be stored at -20°C or lower upon receipt.
    2. -20°C for 12 months under sterile conditions.
  • Notes

    RNA prepared using in vitro transcription and cap analog should be purified prior to use and resuspended in nuclease-free water. EDTA and salts should not be present in the solution.

    Heating the RNA at 65°C for 5 minutes prior to incubation with the enzyme removes secondary structure on the 5´ end of the transcript. Extend time to 10 minutes for transcripts with known highly structured 5´ ends.

Bioactivity

One unit is defined as the amount of enzyme required to methylate 10 pmoles of 80 nt long capped RNA transcript in 1 hour at 37°C.

  • Clinical and Translational Updates

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