Acrobiosystems for English
icon_bulk_orderBulk inquiry/Quick order
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Protein > IL-18 > IL8-H5114

Human IL-18 Protein, premium grade

Order Now

  • Synonym
    IL-18,Interleukin-18,IL18,IL-1 gamma,IGIF,IL1F4,IL-1F4
  • Source
    Human IL-18 Protein, premium grade(IL8-H5114) is expressed from E. coli cells. It contains AA Tyr 37 - Asp 193 (Accession # Q14116-1).
    Human IL-18 Protein, premium grade (IL8-H5114), designed for preclinical stage, has the same activity and performance with GMP Human IL-18 Protein, which enables a seamless transition from preclinical development to clinical phases. Premium Grade product offer a cost efficient alternative of GMP Grade products for the early development phase when safety of raw materials is not top priority. By using Premium Grade products in early development phase, you can transition easily into clinical and commercial phase without need to revalidate the raw materials and modify manufacturing process.
  • Molecular Characterization
    IL-18 Structure

    This protein carries no "tag".

    The protein has a calculated MW of 18.2 kDa. The protein migrates as 16 kDa±3 kDa under reducing (R) condition, and 16 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin
    Less than 0.01 EU per μg by the LAL method.
  • Host Cell Protein
    <0.5 ng/µg of protein tested by ELISA.
  • Host Cell DNA
    <0.02 ng/μg of protein tested by qPCR.
  • Sterility
  • Mycoplasma
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 24 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.

Human IL-18 Protein, premium grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-Bioactivity CELL BASE

Human IL-18 Protein, premium grade (Cat. No. IL8-H5114) stimulates secretion of IFN-γ by KG-1 cells. The specific activity of Human IL-18 Protein, premium grade is > 3.00ⅹ10^6 IU/mg, which is calibrated against WHO Reference Reagent Interleukin-18 (Human rDNA derived) (NIBSC code: 03/200) (QC tested).


Immobilized Human IL-18 Protein, premium grade (Cat. No. IL8-H5114) at 5 μg/mL (100 μL/well) can bind Human IL-18BP, Fc Tag (Cat. No. ILP-H5253) with a linear range of 0.6-20 ng/mL (QC tested).

 IL-18 SPR

Biotinylated Human IL-18 R1, Fc,Avitag (Cat. No. IL1-H82F9) captured on Protein A Chip can bind Human IL-18 Protein, premium grade (Cat. No. IL8-H5114) with an affinity constant of 14.2 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

 IL-18 SPR

Human IL-18BP, Fc Tag (Cat. No. ILP-H5253) captured on Protein A Chip can bind Human IL-18 Protein, premium grade (Cat. No. IL8-H5114) with an affinity constant of 0.781 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    Interleukin-18 (IL-18) is a potent proinflammatory cytokine that induces interferon-gamma (IFN-gamma) production from Th1 cells, NK cells and activated macrophages, particularly in the presence of IL-12. IL-18 also functions in developmental regulation of T-lymphocyte helper type I cells and in Fas-mediated cytotoxicity. Suppression of IL-18 activity is being investigated for treatment of chronic inflammatory diseases such as Crohn's disease and rheumatoid arthritis. It acts by inducing heterodimerization of the two subunits of its receptor, IL-18RAlpha and IL-18RBeta shows structural similarity to IL-1.
  • Clinical and Translational Updates

Comments (0)

ETA of in-stock products: 2 business days

Price(USD) : $240.00

Price(USD) : $550.00

Price(USD) : $2800.00

Promotion & Exhibitions

Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Related Products

New Product Launch

Questions & Comments

This web search service is supported by Google Inc.

Call us
Call us
North America:
+1 800-810-0816 (Toll Free)
Asia & Pacific:
+86 400-682-2521
+1 888-377-6111
1 Innovation Way, Newark, DE 19711, USA

Leave a message