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Ready-to-use Human iPSC-Derived Liver Organoids

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  • Product Details
    The Ready-to-use liver organoids are derived form human iPS cells and being cultured for more than 60 days. Organoids generated using Human iPSC-Derived liver organoid feature various types of cells, including hepatocytes, bile duct cells and vascular endothelial cells.
  • Product Specification
    The live organoids are ready-to-use organoids that are delivered in shipping medium and has to go through a 48h recovery process according to instruction.
  • Storage

    After recovery, please store the organoid in its maintenance medium under the correct incubation condition and medium changing process.

*Please note that to support full process of organoid culture and maintenance, this product have to be used with Human iPSC-Derived Liver Organoid Expansion and Maintenance Medium (Cat. No.RIPO-RWM010),you can follow this link for product information.
Product Diagram
 - PRODUCT DIAGRAM

Protocol Diagram of liver organoid differentiation.

Validation Data
Orgnaoid Histology
 - ORGNAOID HISTOLOGY

H&E Staining of liver organoid.

Marker Expression
 - MARKER EXPRESSION

The liver organoids show expression of hepatocytes (HNF4a), hepatobiliary tracts (CK19), Albumin (ALB) and vascular endothelial cell (CD31).

 - MARKER EXPRESSION

Pre-mature liver organoids show liver lobules-like structure.

Organoid Activity
 - ORGANOID ACTIVITY

Liver organoids show better performance than HepG2 cell line in terms of the expression of Albumin, CYP3A4, CYP1A2, CYP2C9, CYP7A1 and BSEP.

 - ORGANOID ACTIVITY

Liver organoid show response to Rifampicin treatment as indication by the increase fluorescence intensity of CYP3A4.

  • Clinical and Translational Updates

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