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Your Position: Home > Immune Checkpoint Proteins > LAG-3 > Human LAG-3 / CD223 Protein, His Tag

Human LAG-3 / CD223 Protein, His Tag

  • Synonym
    LAG3,CD223,FDC
  • Source
    Human LAG-3, His Tag (LA3-H5222) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Leu 450 (Accession # P18627).
    Predicted N-terminus: Leu 23
  • Molecular Characterization
    Online(Leu 23 - Leu 450) P18627

    This protein carries a polyhistidine tag at the C-terminus, and has a calculated MW of 47.1 kDa. The predicted N-terminus is Leu 23. The reducing (R) protein migrates as 55-60 kDa in SDS-PAGE due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;

    2. -80°C for 6 months under sterile conditions after reconstitution.

SDS-PAGE
Human LAG-3, His Tag (Cat. No. LA3-H5222) SDS-PAGE gel

Human LAG-3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Human LAG-3, His TagHuman LAG-3, His Tag (Cat. No. LA3-H5222) ELISA bioactivity

Immobilized Anti-Human LAG3 MAb, Human IgG1 at 2μg/mL (100 μL/well) can bind Human LAG-3, His Tag (Cat. No. LA3-H5222) with a linear range of 1-6 ng/mL (QC tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Anti-LAG-3 MAb (Human IgG1) captured on CM5 chip via Anti-Human IgG Fc antibodies surface, can bind Human LAG-3, His Tag (Cat. No. LA3-H5222) with an affinity constant of 4.57 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Anti-LAG-3 MAb (Human IgG1) on AHC Biosensor, can bind Human LAG-3, His Tag (Cat. No. LA3-H5222) with an affinity constant of 7.47 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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Price(USD) : $341.00

Price(USD) : $2398.00

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