This protein carries a polyhistidine tag at the C-terminus, and has a calculated MW of 47.1 kDa. The predicted N-terminus is Leu 23. The reducing (R) protein migrates as 55-60 kDa in SDS-PAGE due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human LAG-3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Anti-LAG-3 MAb (Human IgG1) captured on CM5 chip via Anti-Human IgG Fc antibodies surface, can bind Human LAG-3, His Tag (Cat. No. LA3-H5222) with an affinity constant of 4.57 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Loaded Anti-LAG-3 MAb (Human IgG1) on AHC Biosensor, can bind Human LAG-3, His Tag (Cat. No. LA3-H5222) with an affinity constant of 7.47 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Authors: Zhang Wang et al.
Journal: US20210017279A1 2021
Authors: NS Wilson, DA Savitsky, SM Jennings
Journal: US10844119B2 2020
Application: Flow cytometry
Authors: R Stafford, et al
Journal: US20190330336A1 2019
Authors: X Yu, et al
Journal: MAbs 2019
Authors: C Rothe, et al
Journal: US20190010231A1 2019
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $341.00
Price(USD) : $2398.00
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