Acrobiosystems for English
icon_bulk_orderBulk inquiry/Quick order
0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Kits > - > ALK-A002

APC Antibody Labeling Kit

For research use only.

Order Now

  • Application

    APC Antibody Labeling Kit uses a simple and quick process for APC conjugation of antibody. The conjugated antibody can be used straight away without further purification step and antibody loss.

    It is for research use only.

  • Background
    Allophycocyanin (APC) is an intensely bright phycobiliprotein which has an excitation wavelength of 640 nm and an emission wavelength of 661nm. APC Antibody Labeling Kit provides a rapid and simple method to covalently link via the interchain disulfide bonds of the antibody.
  • Assay Principles
    APC Antibody Labeling Kit provides a rapid and simple method for achieving covalently coupling of APC and antibody molecules. The interchain disulfide bonds of the antibody can be modified to generate active cysteine residues, which are then conjugated with the preactivated APC to obtain the APC-antibody conjugate.

    - Assay Principles

    Materials Provided
    IDComponentsSize
    ALK02-C01Modifier reagent1 vial
    ALK02-C02Activated PE1 vial
    ALK02-C03Quencher reagent1 vial
  • Storage
    1. Unopened kit should be stored at -20℃ upon receiving.

    2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

FACS Data Please refer to DS document for the assay protocol.
 - FACS

2.5e5 RC222 cells (anti-CD19 CAR-293 cells, G4S linker is used in CARs) and jurkat cells (with expression of the CD3 antigen) were stained with 100μL of 1μg/mL APC labeled antibody, and analyzed with FACS.

The results show ALK-A002 can be used for different antibodies conjugation.

Bioactivity-FACS Please refer to DS document for the assay protocol.
 - FACS

Binding signal of products prepared by different size of APC antibody labeling Kit (ALK-A002). The result shows very high consistency (QC tested).

 - FACS

The binding signal of APC labeled anti-FMC63 antibody using ALK-A002 Kit was higher than the other competitor kit (Routinely tested).

 - FACS

The binding signal of APC labeled anti-CD3 antibody using ALK-A002 Kit was higher than the other competitor kit (Routinely tested).

  • Clinical and Translational Updates

Comments (0)


ETA of in-stock products: 2 business days

Price(USD) : $295.00

Price(USD) : $695.00

Price(USD) : $995.00

Promotion & Exhibitions



Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Related Products

New Product Launch

Questions & Comments

This web search service is supported by Google Inc.

totop
Call us
Call us
North America:
+1 800-810-0816 (Toll Free)
Asia & Pacific:
+86 400-682-2521
Fax
Fax
+1 888-377-6111
Address
Address
1 Innovation Way, Newark, DE 19711, USA

Leave a message