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Assay Type | FRET |
Analyte | RNase A |
Format | 96T/480T |
Regulatory Status | RUO |
Sensitivity | 0.03125 pg |
Standard Curve Range | 0.03125 pg-2 pg |
Assay Time | 30 min |
Suitable Sample Type | For the quantitative determination of RNase A in the environment, some biological materials, common molecular biological reagents such as reaction buffers, enzymes such as reverse transcriptase and RNA polymerase, and buffers for RNA purification and storage |
Sample volume | 10 μL & 80 μL |
RNase Activity Assay Kit (Fluorescence) is based on the FRET method and is used to measure the presence of RNase in buffers, reagents, and other components within 30 minutes. It is designed to provide a rapid, highly sensitive, and quantitative universal detection tool for identifying RNase contamination that may affect experiments. Moreover, the DNase and RNase Activity Assay Kit (Fluorescence) has been designed to work seamlessly together for the simultaneous quantitative detection of DNases and RNases in a single sample.
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
1. Unopened kit should be stored at -25~-15℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 3 months from the date of opening.
Items | Size （96tests） | Size（480tests） |
RNase Substrate | 2 nmol | 10 nmol |
10X Reaction Buffer for RNase | 10 mL | 10 mL |
RNase A (10μg/mL) | 100 μL | 500 μL |
TE Buffer (pH 7.0) | 1.5 mL | 6 mL |
Nuclease-free Water | 10 mL | 50 mL |
The RNase Activity Assay Kit (Fluorescence) is based on a fluorophore-labeled RNA substrate. When the sample does not contain RNase activity, the substrate is stable and does not produce a fluorescent signal; when the sample contains RNase activity, the substrate is degraded, resulting in a gradual enhanced fluorescence signal, the rate of increase in fluorescence signal is positively correlated with the number and activity of enzymes. Use a fluorescence microplate reader to measure at the wavelength of ex/em= 490/520 nm to determine whether the sample is contaminated by RNase.
