Save Product
Product Details
Materials Provided
IDComponentsSizeRAS020-C01Pre-coated with Anti-SARS-CoV-2 Spike Protein Antibody Microplate1 plateRAS020-C02SARS-CoV-2 Spike Protein10 μgRAS020-C03Biotin-Anti-SARS-CoV-2 Spike Protein Antibody10 μgRAS020-C04Streptavidin-HRP10 μgRAS020-C0510xWashing Buffer50 mLRAS020-C06Dilution Buffer50 mLRAS020-C07Substrate Solution12 mLRAS020-C08Stop Solution7 mLApplication
This kit is developed for detecting SARS-CoV-2 Spike Protein in vaccine samples, which can meet the needs of vaccine developers to establish antigen quantification methods for preclinical evaluation, vaccine production and quality control, and realize accurate quantification of vaccine antigen contents for COVID-19 vaccines of all technological platforms, multivalent vaccines, and VOC-specific boosters.
It is for research use only.
Reconstitution
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
Storage
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Assay Principles
This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of SARS-CoV-2 Spike Protein. The kit consists of microplate pre-coated with Anti-SARS-CoV-2 Spike protein Antibody, an SARS-CoV-2 Spike Protein as Control, an biotin-Anti-SARS-CoV-2 Spike protein Antibody, HRP-Streptavidin and buffers.
Your experiment will include 6 simple steps:
a) All reagents were returned to room temperature(20°C-25°C) before use.
b) Add your sample to the plate, take the SARS-CoV-2 Spike protein as Control sample. The samples and Control sample are diluted by Dilution Buffer.
c) Add a diluted Secondary antibody biotin-Anti-SARS-CoV-2 Spike protein Antibody to the plate. The Secondary antibody is diluted by Dilution Buffer.
d) Add a diluted Streptavidin-HRP to the plate.
e) Wash the plate and add TMB or other colorimetric HRP substrate.
f) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.
ACRO Quality Management System
Customers Also Viewed
Performance Data
Typical Data
Please refer to DS document for the assay protocol.
This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of SARS-CoV-2 Spike protein. The kit consists of pre-coated Anti-SARS-CoV-2 Spike Protein Antibody Microplate, SARS-CoV-2 Spike protein as Control, biotin-Anti-SARS-CoV-2 Spike protein antibody and HRP-Streptavidin. Detection is performed using HRP-Streptavidin with sensitivity of 200 pg/mL.
SARS-CoV-2 Spike Protein Titer Assay Kit (Cat.No.RAS-A020) can potently to detect the all SARS-CoV-2 Variants of Concern (VOCs), including Alpha (Cat.No.SPN-C52H6), Beta (Cat.No.SPN-C52Hk), Gamma (Cat.No.SPN-C52Hg) and Delta (Cat.No.SPN-C52He).
SARS-CoV-2 Spike Protein Titer Assay Kit (Cat.No.RAS-A020) can potently to detect the all SARS-CoV-2 Variants of Concern (VOCs), including Omicron (Cat.No.SPN-C52Hz).
Customer Reviews Writing Reviews
Recent Advances
Drug Development Progress- English Name：
Coronavirus spike glycoprotein
- Category：
- Approved Drugs：
15 Details
- Drugs in Clinical Trials：
88 Details
- Highest Development Stage：
Approved
Contact Us
[North America]: +1 800-810-0816
[Switzerland]: +41 800 040 012
[Asia & Pacific]: +86 400-682-2521
Drug Candidate Licensing
