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Human iPSC-Derived Intestinal Organoids are differentiated from hESC or iPSCs using Human iPSC-Derived Intestinal Organoid Differentiation Kit. Intestinal organoids are three-dimensional in vitro models with a cellular composition and structural organization that is representative to the human Intestinal regions. The Ready-to-use Intestinal organoids are derived form human iPS cells and being cultured for more than 60 days. Organoids generated using Human iPSC-Derived Intestinal Organoid feature various types of cells, including Intestinal epithelium cells, mesenchyme cells, enterocytes, Paneth cells, goblet cells, etc. These Intestinal organoids show intestine crypt like structure, Villi and Microvilli like structure. These Intestinal organoids show normal intestinal function validated by the absorption of fatty acid and glucose. The organoid will be QC in day 60, and we start to send out from day 70. It's suggested to finish all the experiments before day 120. During transport, the organoids are kept in a low metabolic near resting state, and the timing restarts after the completion of the resuscitation step.
The Intestinal organoids are ready-to-use organoids that are delivered in shipping medium and has to go through a 48h recovery process according to instruction.
After recovery, please store the organoid in its maintenance medium under the correct incubation condition and medium changing process.