Save Product
Product Details
Product Details
HRV 3C Protease is highly specific for the recognition sequence Leu-Glu-Val-Leu-Phe-Gln-↓-Gly-Pro and cleaves after the glutamine residue.
Materials Provided
ComponentsSizeHRV-3C Protease Cleavage Enzyme, GST Tag1000 U/5000 UHRV GST-3C Cleavage Control Protein100 ug10× HRV-3C Cleavage Buffer25 mLApplication
Removal of purification tags from fusion proteins.
Unit Definition
One unit will cleave >95% of 100 µg control fusion protein in 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 at 4℃ for 16 h.
Purity
>95% as determined by SDS-PAGE.
Enzyme Activity
>1 U/μL
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0 with trehalose as protectant.
Contact us for customized product form or formulation.
Shipping and Storage
This product is shipped at ambient temperature.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state.
- -70°C for 12 months under sterile conditions after reconstitution.
- *The 10× Reaction Buffer provided in this kit contains DTT, a sulfhydryl reducing agent essential for maintaining enzyme activity. When employing the 10× Reaction Buffer exposed to prolonged storage or repeated freeze-thaw cycles, fresh 10 mM DTT should be supplemented to the Reaction Buffer to ensure maximal enzymatic activity, as oxidative degradation of DTT progressively compromises its reducing capacity.
Customers Also Viewed
Performance Data
SDS-PAGE
The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity
>1 Units/µg. One unit will cleave >95% of 100 µg control fusion protein in 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 at 4℃ for 16 h (QC tested).
Customer Reviews Writing Reviews
T***n2022-12-10
LikesI'm using this product in my protein purification to cleave GST tag . Commercially available enzymes from other companies are expensive , not working properly. HRV3C from Acros Biosystem works great and very cheap price. I'm really happy to use this product. otherwise , I have to spent a lot of money to get this enzyme. Thanks, Thiru
Recent Advances
Drug Development Progress- English Name：
Picornain 3C protease
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
0 Details
- Highest Development Stage：
Preclinical
Contact Us
[North America]: +1 800-810-0816
[Switzerland]: +41 800 040 012
[Asia & Pacific]: +86 400-682-2521
Drug Candidate Licensing
