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Product Details
Product Details
Bst DNA Polymerase is a recombinant form of Bacillus stearothermophilus DNA Polymerase, Large Fragment produced in Escherichia coli. Bst DNA Polymerase contains 5´→3´ DNA polymerase activity with either DNA or RNA templates and strong strand displacement activity, but lacks 5´→3´ and 3´→5´ exonuclease activity.
Materials Provided
ComponentsSizeBst DNA Polymerase1600 U/8000 UBst DNA Polymerase 10× Reaction Buffer1.5 mL100 mM MgSO41.5 mLApplication
Isothermal amplification (LAMP, RT-LAMP).
DNA sequencing of rich GC sequence.
Rapid sequencing of DNA templates (ng).Unit Definition
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 65°C.
Purity
>90% as determined by SDS-PAGE.
Enzyme Activity
>8 U/μL
Formulation
Supplied as 0.2 μm filtered solution in 10 mM Tris-HCl, pH7.1 with glycerol as protectant.
Contact us for customized product form or formulation.
Shipping and Storage
This product is supplied and shipped on dry ice.
This product is stable after storage at:
- The product MUST be stored at -20°C or lower upon receipt.
- -20°C for 3 months under sterile conditions.
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Performance Data
SDS-PAGE
The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity
One unit is defined at the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 65°C (QC tested).
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