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Human proA Protein, His Tag (Nanodisc-pro)

Cat. No. / Size
Price
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APO-H5143-30ug
$125.00
In Stock - Delivery in 1-2 business days
APO-H5143-150ug
$410.00
In Stock - Delivery in 2-3 weeks
APO-H5143
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Product Details
Performance Data
Reviews & Citations
Resources
  • Product Details
  • Performance Data
  • Reviews & Citations
  • Important Statement
  • Resources
Application
  • Cell based assay (FACS or functional assay)
  • Affinity testing (SPR / BLI)
  • Screening (ELISA)
Synonyms

proA

Source

Nanodisc-pro Human proA Protein, His Tag (APO-H5143) is expressed from E. coli system.

Molecular Characterization

This protein carries a polyhistidine tag at the N-terminus. This protein is used together with nanodisc-pro protein as isotype control.

Structure

Nanodisc-pro membrane protein is wrapped by a piece of cell membrane and scaffold, the info of scaffold can be found in above red text "isotope type control...".

Synthesis Process

Nanodisc-pro membrane protein extraction is a gentle, non-disruptive process that directly isolates full-length transmembrane proteins together with their surrounding native phospholipids from cellular membranes, without breaking or disturbing the original membrane structure. This preserves the membrane protein's natural conformational landscape and native lipid environment, yielding stable nanodisc that retain cellular phospholipids, with fully preserved biological activity—making them ideal for cell-based assays such as FACS and binding assays in antibody discovery and screening (ELISA), etc.

Purity

>90% as determined by SDS-PAGE.

Formulation

Supplied as 0.2 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with glycerol as protectant.

Contact us for customized product form or formulation.

Shipping and Storage

This product is supplied and shipped on dry ice.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • The product MUST be stored at -70°C or lower upon receipt;
  • -70°C for 12 months under sterile conditions.
ACRO Quality Management System

Background

Nanodiscs are artificially constructed lipoprotein complexes that mimic the lipid bilayer environment found in natural cell membranes. Composed of a phospholipid bilayer encircled by amphipathic membrane scaffold proteins (MSPs), they form a discoidal structure. This design enables stable encapsulation of membrane proteins or lipid-associated antigens, preserving their native conformation and biological activity, while also providing good water solubility and monodispersity. Nanodiscs are widely used in membrane protein functional studies, vaccine development, drug delivery, and antigen presentation.