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Your Position: Home > Protein > IL-1 beta > ILB-H4110

Human IL-1 beta / IL-1F2 Protein, premium grade

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  • Synonym
    IL1B,IL-1BETA,IL1F2,IL-1β
  • Source
    Human IL-1 beta, premium grade(ILB-H4110) is expressed from E. coli cells. It contains AA Ala 117 - Ser 269 (Accession # P01584-1 ).
    Predicted N-terminus: Met
    Human IL-1 beta, premium grade (ILB-H4110), designed for preclinical stage, has the same activity and performance with GMP Human IL-1 beta (GMP-ILBH16), which enables a seamless transition from preclinical development to clinical phases. Premium Grade product offer a cost efficient alternative of GMP Grade products for the early development phase when safety of raw materials is not top priority. By using Premium Grade products in early development phase, you can transition easily into clinical and commercial phase without need to revalidate the raw materials and modify manufacturing process.
  • Molecular Characterization
    IL-1 beta Structure

    This protein carries no "tag".

    The protein has a calculated MW of 17.5 kDa. The protein migrates as 17 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 0.02 EU per μg by the LAL method.
  • Sterility
    The sterility testing was performed by membrane filtration method.
  • Mycoplasma
    Negative.
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-1 beta SDS-PAGE

Human IL-1 beta, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
IL-1 beta MALS images

The purity of Human IL-1 beta, premium grade (Cat. No. ILB-H4110) is more than 95% and the molecular weight of this protein is around 15-23 kDa verified by SEC-MALS.

Bioactivity-Bioactivity CELL BASE
 IL-1 beta CELL

Human IL-1 beta, premium grade (Cat. No. ILB-H4110) stimulates secretion of IFN-γ by NK-92 human natural killer lymphoma cells stimulated with 0.5 ng/mL Human IL-12B&IL-12A Heterodimer Protein, His Tag&Flag Tag (Cat. No. IL2-H4210). The specific activity of Human IL-1 beta, premium grade is > 7.0 × 10^7 IU/mg, which is calibrated against human IL-1 beta WHO International Standard (NIBSC code: 86/680) (Routinely tested).

Bioactivity-ELISA
 IL-1 beta ELISA

Immobilized Human IL-1 beta, premium grade (Cat. No. ILB-H4110) at 10 μg/mL (100 μL/well) can bind Human IL-1 RII, Fc Tag (Cat. No. IL2-H4256) with a linear range of 8-63 ng/mL (QC tested).

Bioactivity-SPR
 IL-1 beta SPR

Human IL-1 RII, Fc Tag (Cat. No. IL2-H4256) captured on CM5 chip via anti-human IgG Fc antibodies surface can bind Human IL-1 beta, premium grade (Cat. No. ILB-H4110) with an affinity constant of 0.622 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

  • Background
    Interleukin-1 beta (IL-1β) is also known as catabolin, is a cytokine protein that in humans is encoded by the IL1B gene. IL-1β precursor is cleaved by caspase 1 (interleukin 1 beta convertase). Cytosolic thiol protease cleaves the product to form mature IL-1 beta.
    IL1β are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL1α and IL1β are regulated independently, they bind to the same receptor and exert identical biological effects.
    IL-1β is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
  • Clinical and Translational Updates

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