This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 70.2 kDa. The protein migrates as 110-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 12 months under sterile conditions after reconstitution.
Human Her2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human Her2, His Tag (Cat. No. HE2-H5225) was more than 90% and the molecular weight of this protein is around 80-95 kDa verified by SEC-MALS.
Immobilized Human Her2, His Tag (Cat. No. HE2-H5225) at 1 μg/mL (100 μL/well) can bind Pertuzumab Biosimilar with a linear range of 0.2-4 ng/mL (QC tested).
Herceptin (Trastuzumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human Her2, His Tag (Cat. No. HE2-H5225) with an affinity constant of 1.07 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Loaded Herceptin (Trastuzumab) on AHC Biosensor, can bind Human Her2, His Tag (Cat. No. HE2-H5225) with an affinity constant of 0.825 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Authors: Spanov, Baubek et al.
Journal: Journal of chromatography 2021
Application: Binding Assay
Authors: Ollier R, et al
Journal: MAbs 2019
Authors: E Zhukovsky, et al
Journal: US20190153104A1 2019
Authors: Hao Y, et al
Journal: PLoS One 2019
Application: Protein purification
Authors: S Shukla, et al
Journal: Adv Ther 2019
Authors: Hussack G, et al
Journal: BMC Res Notes 2018
Application: Immunization & SPR
Authors: J Rohloff et al.
Journal: US9938314B2 2018
Authors: S Chatterjee, et al.
Journal: US20180171028A1 2018
Authors: Anami Y, et al.
Journal: Nat Commun 2018
Authors: Zhou Z, et al.
Journal: Bioorg Med Chem 2018
Authors: Wang JH, et al.
Journal: Mol Cancer Ther 2018
Authors: Nath N, et al.
Journal: J Immunol Methods 2017
Authors: Shinozaki N, et al.
Journal: Sci Rep 2017
Application: Rabbit immunization
Authors: Elena Ambrosetti, et al.
Journal: ACS Omega 2017
Application: Multiplex detection & SPR & AFM & ELISA
Authors: Ken S. Rosenthal, et al.
Journal: J Immunol Res 2017
Authors: Dakshinamurthy P, et al.
Journal: Biologicals 2017
Authors: S Shukla, et al.
Journal: Biomaterials 2016
Authors: Chen J, et al.
Journal: Agilent 2016
Authors: Natalya D Bodyak, et al.
Journal: US20150366987A1 2015
Authors: Stojanović I, et al.
Journal: Anal Biochem 2015
Authors: NDong C, et al.
Journal: PLoS One 2015
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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