>Immune Checkpoint Proteins
>Biotinylated Human PD-L1 /CD274 Protein, C-Fc, His & AVI Tagged,Superior Sensitivity
This protein carries a human IgG1 Fc fragment at the C-terminus, followed by a polyhistidine tag. The Avi tag (Avitag™) is inserted in-between the Fc and his tags. The protein has a calculated MW of 53.2 kDa. The protein migrates as 66-75 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 10 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
Biotinylated Human PD-L1, His Tag, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human PD-1, Fc Tag (Cat. No. PD1-H5257) at 2 μg/mL (100μL/well) can bind Biotinylated Human PD-L1, His Tag & Fc tag (Cat. No. PD1-H82F3) with a linear range of 0.01-0.25 μg/mL (QC tested).
Flow Cytometry assay shows that recombinant Biotinylated human PD-L1, His Tag, Fc Tag, Avi Tag (Cat.No. PD1-H82F3) can bind to 293 cell overexpressing human PD-1. The concentration of PD-L1 used is 1 μg/ml (Routinely tested).
FACS analysis shows that the binding of Biotinylated human PD-L1, His Tag, Fc Tag, Avi Tag (Cat.No. PD1-H82F3) to 293 overexpressing PD-1 was inhibited by increasing concentration of neutralizing anti-PD-1 antibody. The concentration of PD-L1 used is 2μg/ml. The IC50 is 0.64 μg/ml (Routinely tested).
Immobilized cell surface PD-1(5x104 of cells per well) can bind Biotinylated human PD-L1, His Tag, Fc Tag, Avi Tag (Cat.No. PD1-H82F3) with an EC50 of 0.082ug/ml (Routinely tested).
The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.
Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $385.00
Price(USD) : $1518.00
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