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Your Position: Home > Immune Checkpoint Proteins > PD-L1 > Biotinylated Human PD-L1 /CD274 Protein, C-Fc, His & AVI Tagged,Superior Sensitivity

Biotinylated Human PD-L1 / B7-H1, His Tag & Fc Tag, Avi Tag (Avitag™)

  • Synonym
    PD-L1,CD274,B7-H1,PDCD1L1,PDCD1LG1
  • Source
    MABSol® Biotinylated Human PD-L1 / B7-H1, His Tag & Fc Tag (PD1-H82F3) is expressed from human HEK293 cells. It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1).
    Predicted N-terminus: Phe 19
  • Molecular Characterization
    PD-L1(Phe 19 - Arg 238) NP_054862.1

    This protein carries a human IgG1 Fc fragment at the C-terminus, followed by a polyhistidine tag. The Avi tag (Avitag™) is inserted in-between the Fc and his tags.  The protein has a calculated MW of 53.2 kDa. The protein migrates as 60-75 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arg, 10 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Biotinylated Human PD-L1, His Tag & Fc Tag (Cat. No. PD1-H82F3) SDS-PAGE gel

Biotinylated Human PD-L1, His Tag & Fc Tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Biotinylated Human PD-L1, His Tag & Fc TagBiotinylated Human PD-L1, His Tag & Fc Tag (Cat. No. PD1-H82F3) ELISA bioactivity

Immobilized Human PD-1, Fc Tag (Cat. No. PD1-H5257) at 2 μg/mL (100μL/well) can bind Biotinylated Human PD-L1, His Tag & Fc tag (Cat. No. PD1-H82F3) with a linear range of 0.01-0.25 μg/mL (QC tested).

Biotinylated Human PD-L1, His Tag & Fc TagBiotinylated Human PD-L1, His Tag & Fc Tag (Cat. No. PD1-H82F3) ELISA bioactivity

Immobilized Human B7-1, mouse IgG2a Fc Tag, low endotoxin (Cat. No. B71-H52A4) at 5 μg/mL (100 μL/well) can bind Biotinylated Human PD-L1, His Tag, Fc Tag (Cat. No. PD1-H82F3) with a linear range of 0.02-0.625 μg/mL (Routinely tested).

FACS assay shows that recombinant Biotinylated FACS

Flow Cytometry assay shows that recombinant Biotinylated human PD-L1, His Tag, Fc Tag, Avi Tag (Cat.No. PD1-H82F3) can bind to 293 cell overexpressing human PD-1. The concentration of PD-L1 used is 1ug/ml  (Routinely tested).

FACS assay shows that recombinant Biotinylated FACS

FACS analysis shows that the binding of Biotinylated human PD-L1, His Tag, Fc Tag, Avi Tag (Cat.No. PD1-H82F3) to 293 overexpressing PD-1 was inhibited by  increasing concentration of neutralizing  anti-PD-1 antibody. The concentration of PD-L1 used is 2ug/ml. The IC50 is 0.64 ug/ml (Routinely tested).

Human FcRn Heterodimer Protein Cell_Base

Immobilized cell surface PD-1(5x104 of cells per well) can bind Biotinylated human PD-L1, His Tag, Fc Tag, Avi Tag (Cat.No. PD1-H82F3) with an EC50 of 0.082ug/ml (Routinely tested).

  • Background
    Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.
  • References
  • Limited Use License

    The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

    Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1380.00

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