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Your Position: Home > All Other Proteins > CD3 epsilon > Cynomolgus CD3 epsilon Protein, Fc,His Tag

Cynomolgus CD3 epsilon Protein, Fc,His Tag

  • Synonym
    FLJ18683,T3E,TCRE,CD3E,CD3-epsilon
  • Source
    Cynomolgus CD3 epsilon, Fc,His Tag (CDE-C5254) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asp 117 (Accession # Q95LI5).
    Predicted N-terminus: Gln 22
  • Molecular Characterization
    Online(Gln 22 - Asp 117) Q95LI5

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 38.3 kDa. The protein migrates as 43-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Cynomolgus CD3 epsilon, Fc,His Tag (Cat. No. CDE-C5254) SDS-PAGE gel

Cynomolgus CD3 epsilon, Fc,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Cynomolgus CD3 epsilon, Fc,His TagCynomolgus CD3 epsilon, Fc,His Tag (Cat. No. CDE-C5254) ELISA bioactivity

Immobilized SP34-2 at 2 μg/mL (100 μL/well) can bind Cynomolgus CD3 epsilon, Fc,His Tag (Cat. No. CDE-C5254) with a linear range of 0.2-3 ng/mL (Routinely tested).

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Price(USD) : $1980.00

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