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Your Position: Home > Fc Receptor Proteins > Fc gamma RI / CD64 > CD4-M5227

Mouse Fc gamma RI / CD64 Protein, His Tag (MALS & BLI verified)

  • Synonym
    FCGR1A,FCG1,FCGR1,IGFR1,CD64,CD64A,FCRI,FCGR-1,IGFR-1,Fcr,Fcgr,Fc receptor,Fc gamma receptor
  • Source
    Mouse CD64, His Tag (CD4-M5227) is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Pro 297 (Accession # P26151-1).
    Predicted N-terminus: Glu 25
  • Molecular Characterization
    Online(Glu 25 - Pro 297) P26151-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 32.3 kDa. The protein migrates as 40-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mouse CD64, His Tag (Cat. No. CD4-M5227) SDS-PAGE gel

Mouse CD64, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Mouse CD64, His Tag (Cat. No. ) MALS images

The purity of Mouse CD64, His Tag (Cat. No. CD4-M5227) was more than 90% and the molecular weight of this protein is around 50-60 kDa verified by SEC-MALS.

Bioactivity-ELISA
Mouse CD64, His TagMouse CD64, His Tag (Cat. No. CD4-M5227) ELISA bioactivity

Immobilized Anti-CD3 epsilon MAb, Mouse IgG2a (Cat. No. CDE-M120a) at 2 μg/mL (100 μL/well) can bind Mouse CD64, His Tag (Cat. No. CD4-M5227) with a linear range of 1-8 ng/mL (QC tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Captured Mouse CD64, His Tag (Cat. No. CD4-M5227) on NTA-Series S sensor chip can bind Herceptin® (Trastuzumab) with an affinity constant of 63.8 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Herceptin on Protein A Biosensor, can bind Mouse CD64, His Tag (Cat. No. CD4-M5227) with an affinity constant of 31 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Citations
  • Background
    Receptors that recognize the Fc portion of IgG are divided into three groups designated Fc gamma RI, RII, and RIII, also known respectively as CD64, CD32, and CD16. Fc gamma RI binds IgG with high affinity and functions during early immune responses. Fc gamma RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. High affinity immunoglobulin gamma Fc receptor I is also known as FCGR1A, FCG1, FCGR1, CD64 and IGFR1, is a type of integral membrane glycoprotein that binds monomeric IgG-type antibodies with high affinity, which belongs to the immunoglobulin superfamily or FCGR1 family. FCGR1A / CD64 contains 3 Ig-like C2-type (immunoglobulin-like) domains. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:0 Details
  • Latest Research Phase:Discontinued

Datasheet & Documentation

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