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Product Details
Synonyms
PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1
Source
Cynomolgus / Rhesus macaque PD-L1, Fc Tag (PD1-C5253) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # F6VEW6-1). In the region Phe 19 - Arg 238, the amino acid sequences of Cynomolgus and Rhesus macaque PD-L1 are identical.
Predicted N-terminus: Phe 19
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 51.8 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Cynomolgus / Rhesus macaque PD-L1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Cynomolgus / Rhesus macaque PD-L1, Fc Tag (Cat. No. PD1-C5253) is more than 85% and the molecular weight of this protein is around 110-140 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Cynomolgus PD-1, His,Avitag (Cat. No. PD1-C82E6) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind Cynomolgus / Rhesus macaque PD-L1, Fc Tag (Cat. No. PD1-C5253) with a linear range of 1-5 ng/mL (Routinely tested).
Protocol
Immobilized Cynomolgus / Rhesus macaque PD-L1, Fc Tag (Cat. No. PD1-C5253) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus PD-1 Protein, Fc,Avitag (Cat. No. PD1-C82F7) with a linear range of 0.004-0.125 μg/mL (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Cynomolgus / Rhesus macaque PD-L1, Fc Tag (Cat. No. PD1-C5253) on Protein A Biosensor, can bind Cynomolgus PD-1, His Tag (Cat. No. PD1-C52H5) with an affinity constant of 4.2 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
Loaded Cynomolgus PD-1, His Tag (Cat. No. PD1-C52H5) on HIS1K Biosensor, can bind Cynomolgus / Rhesus macaque PD-L1, Fc Tag (Cat. No. PD1-C5253) with an affinity constant of 28.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
Background
Recent Advances
Drug Development Progress- English Name：
Programmed death-ligand 1
- Category：
- Approved Drugs：
12 Details
- Drugs in Clinical Trials：
176 Details
- Highest Development Stage：
Approved
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