This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 67.4 kDa. The protein migrates as 66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human Serum Albumin, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human Serum Albumin, His Tag (Cat. No. HSA-H5220) is more than 90% and the molecular weight of this protein is around 60-75 kDa verified by SEC-MALS.
Human Serum Albumin Protein, His Tag (Cat. No. HSA-H5220) immobilized on CM4 Chip can bind Human FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCN-H52W7) with an affinity constant of 0.381 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Loaded Biotinylated Human FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-H82W7) on SA Biosensor, can bind Human Serum Albumin, His Tag (Cat. No. HSA-H5220) with an affinity constant of 0.647 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
A quantitative Streptococcus pyogenes–human protein–protein interaction map reveals localization of opsonizing antibodies
Authors: Happonen L, et al
Journal: Nat Commun 2019
Application: Reverse affinity-capture
Peripheral protein unfolding drives membrane bending
Authors: Siaw HMH, et al.
Journal: Langmuir 2018
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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