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Your Position: Home > Protein > IL-2 R alpha > ILA-H5251

Human IL-2 R alpha / CD25 Protein, Fc Tag (MALS verified)

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  • Synonym
    IL2RA,CD25,p55,IL2-RA,IL-2-RA
  • Source
    Human IL-2 R alpha Protein, Fc Tag(ILA-H5251) is expressed from human 293 cells (HEK293). It contains AA Glu 22 - Cys 213 (Accession # NP_000408).
    Predicted N-terminus: Glu 22
  • Molecular Characterization
    IL-2 R alpha Structure

    This protein carries a human IgG1 Fc tag at the C-terminus

    The protein has a calculated MW of 48.4 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-2 R alpha SDS-PAGE

Human IL-2 R alpha Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
IL-2 R alpha MALS images

The purity of Human IL-2 R alpha Protein, Fc Tag (Cat. No. ILA-H5251) is more than 90% and the molecular weight of this protein is around 102-124 kDa verified by SEC-MALS.

Bioactivity-ELISA
 IL-2 R alpha ELISA

Immobilized Human IL-2 R alpha Protein, Fc Tag (Cat. No. ILA-H5251) at 2 μg/mL (100 μL/well) can bind Human IL-2, Tag Free with a linear range of 0.4-3 ng/mL (QC tested).

Bioactivity-SPR
 IL-2 R alpha SPR

Human IL-2 R alpha Protein, Fc Tag (Cat. No. ILA-H5251) captured on CM5 chip via anti-human IgG Fc antibody can bind Human IL-2, Tag Free with an affinity constant of 10.1 nM as determined in a SPR assay (Biacore T200) (Routinely tested)

Bioactivity-BLI
 IL-2 R alpha BLI

Loaded Human IL-2 R alpha, Fc Tag (Cat. No. ILA-H5251) on Protein A Biosensor, can bind Human IL-2, Tag Free with an affinity constant of 43.2 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Interleukin-2 receptor subunit alpha (IL2RA) is also known as IL-2R subunit alpha, IL-2-RA, IL2-RA, TAC antigen, p55, CD antigen CD25, is a type I transmembrane glycoprotein. IL2RA is expressed on activated T cells and regulatory T cells, and is capable of binding IL2 with low affinity by itself. However, a ligand-induced high affinity heterotrimeric receptor complex is produced when IL2RA is associated non-covelently with the IL2 receptor beta and gamma chain, and subsequently initiates the intacellular signal pathways such as MAPK or JAK/STAT. On dendritic cells (DC), CD25 has been previously regarded as an activation marker, while both murine and human DC can express CD25, they do not express the beta-chain of the IL-2 receptor, which is indispensable for the execution of IL-2 signaling.
  • Clinical and Translational Updates

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