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Your Position: Home > All Other Proteins > PCSK9 > PC9-R52H4

Rat PCSK9 Protein, His Tag

  • Synonym
    PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9
  • Source
    Rat PCSK9, His Tag (PC9-R52H4) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Gln 691 (Accession # P59996-1).
    Predicted N-terminus: Gln 31
  • Molecular Characterization
    Online(Gln 31 - Gln 691) P59996-1

    This protein carries a polyhistidine tag at the C-terminus. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The pro-peptide and mature chain are associated through non‑covalent interactions and with a calculated MW of 13.9 kDa and 59.3 kDa respectively. The protein migrates as 16 kDa and 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by reduced SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Rat PCSK9, His Tag (Cat. No. PC9-R52H4) SDS-PAGE gel

Rat PCSK9, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Rat PCSK9, His TagRat PCSK9, His Tag (Cat. No. PC9-R52H4) ELISA bioactivity

Immobilized Human LDL R, Strep Tag (Cat. No. LDR-H5281) at 2 μg/mL (100 μL/well) can bind Rat PCSK9, His Tag (Cat. No. PC9-R52H4) with a linear range of 8-63 ng/mL (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human LDL R, Fc Tag (Cat. No. LDR-H5254) on Protein A Biosensor, can bind Rat PCSK9, His Tag (Cat. No. PC9-R52H4) with an affinity constant of 1.61 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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Price(USD) : $260.00

Price(USD) : $1980.00

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Drug Development Status

  • Number of Launched Drugs:2 Details
  • Number of Drugs in Clinical Trials:24 Details
  • Latest Research Phase:Approved

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DMF (Drug Master File)

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