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Your Position: Home > Protein > IL-6 > IL6-H4218

Human IL-6 Protein, premium grade

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  • Synonym
    IL6,Interleukin-6,BSF2,HSF,IFNB2
  • Source
    Human IL-6, premium grade(IL6-H4218) is expressed from human 293 cells (HEK293). It contains AA Val 30 - Met 212 (Accession # NP_000591 ).
    Predicted N-terminus: Val 30
    Human IL-6, premium grade (IL6-H4218), designed for preclinical stage, has the same activity and performance with GMP Human IL-6, which enables a seamless transition from preclinical development to clinical phases. Premium Grade product offer a cost efficient alternative of GMP Grade products for the early development phase when safety of raw materials is not top priority. By using Premium Grade products in early development phase, you can transition easily into clinical and commercial phase without need to revalidate the raw materials and modify manufacturing process.
  • Molecular Characterization
    IL-6 Structure

    This protein carries no "tag"

    The protein has a calculated MW of 20.8 kDa. The protein migrates as 23-24 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

  • Endotoxin
    Less than 0.02 EU per μg by the LAL method.
  • Sterility
    The sterility testing was performed by membrane filtration method.
  • Mycoplasma
    Negative.
  • Purity

    >85% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-6 SDS-PAGE

Human IL-6, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.

Bioactivity-Bioactivity CELL BASE
 IL-6 CELL

Human IL-6, premium grade (Cat. No. IL6-H4218) stimulates proliferation of TF-1 human erythroleukemic cell line. The EC50 for this effect is 0.2856-0.3636 ng/mL (Routinely tested).

 IL-6 CELL

Response to human IL-6 protein (Fold).
The STAT3 (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human IL-6 protein (AcroBiosystems, Cat. No. IL6-H4218). The max induction fold was approximately 100.5 (Routinely tested).

Bioactivity-ELISA
 IL-6 ELISA

Immobilized Human IL-6, premium grade (Cat. No. IL6-H4218) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-6 R alpha, Avitag,His Tag (Cat. No. CD6-H82E8) with a linear range of 4-125 ng/mL (QC tested).

Bioactivity-BLI
 IL-6 BLI

Loaded Human IL-6 R alpha, Fc Tag (Cat. No. ILR-H5259) on Protein A Biosensor, can bind Human IL-6, premium grade(Cat. No. IL6-H4218) with an affinity constant of 35.9 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Interleukin 6 (IL-6) is also known as HGF, BSF2,HSF, IFNB2 and IL-6, originally identified as a B cell differentiation factor, is a multifunctional cytokine that regulates immune responses, hematopoiesis, acute phase responses, and inflammatory reactions.It is secreted by T cells, macrophages , monocytes, fibroblasts,endothelial cells,et.al. to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein. IL-6 is relevant to many disease processes such as diabetes,atherosclerosis, depression,Alzheimers Disease,systemic,lupus erythematosus,prostate cancer and rheumatoid arthritis. Advanced/metastatic cancer patients have higher levels of IL-6 in their blood.Hence there is an interest in developing anti-IL-6 agents as therapy against many of these diseases.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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