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Product Details
Synonyms
CD71, TFRC, TFR, sTfR, TfR1, Trfr, T9, p90
Source
Biotinylated Human Transferrin R, His Tag, primary amine labeling (TFR-H8243) is expressed from human 293 cells (HEK293). It contains AA Cys 89 - Phe 760 (Accession # AAH01188.1). It is the biotinylated form of Human Transferrin R, His Tag.
Predicted N-terminus: His
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 76.0 kDa. The protein migrates as 75-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
Labeling
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human Transferrin R, His Tag, primary amine labeling on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human Transferrin R, His Tag, primary amine labeling (Cat. No. TFR-H8243) is more than 90% and the molecular weight of this protein is around 145-195 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human Transferrin Protein, His Tag (Cat. No. TRN-H52H3) at 2 μg/mL (100 μL/well) can bind Biotinylated Human Transferrin R, His Tag, primary amine labeling (Cat. No. TFR-H8243) with a linear range of 1.2-78 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Monoclonal Anti-CD71 Antibody, Mouse IgG1 (5B5) (Cat. No. CD1-MY2101) captured on CM5 chip via anti-mouse antibodies surface can bind Biotinylated Human Transferrin R, His Tag, primary amine labeling (Cat. No. TFR-H8243) with an affinity constant of 0.876 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Transferrin receptor protein 1
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
15 Details
- Highest Development Stage：
Approved
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