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Your Position: Home > Protein > GPA33 > GP3-H8224

Biotinylated Human GPA33 Protein, His Tag, ultra sensitivity (primary amine labeling)

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Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.

  • Synonym
    GPA33,A33
  • Source
    MABSol® Biotinylated Human GPA33, His Tag, primary amine labeling (GP3-H8224) is expressed from human HEK293 cells. It contains AA Ile 22 - Val 235 (Accession # AAH74830). It is the biotinylated form of Human GPA33, His Tag (GP3-H5224).
    Predicted N-terminus: Ile 22
  • Molecular Characterization
    Online(Ile 22 - Val 235) AAH74830

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 24.4 kDa. The protein migrates as 33-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human GPA33, His Tag, primary amine labeling (Cat. No. GP3-H8224) SDS-PAGE gel

Biotinylated Human GPA33, His Tag, primary amine labeling on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human GPA33, His Tag, primary amine labelingBiotinylated Human GPA33, His Tag, primary amine labeling (Cat. No. GP3-H8224) ELISA bioactivity

Immobilized Biotinylated Human GPA33, His Tag, primary amine labeling (Cat. No. GP3-H8224) at 5 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind Monoclonal Anti-Human GPA33 Antibody, Human IgG1 with a linear range of 0.3-1 ng/mL (QC tested).

  • Background
    Glycoprotein A33 (GPA33) is also known as Cell surface A33 antigen, is a single-pass type I membrane protein which is expressed in normal gastrointestinal epithelium and in 95% of colon cancers. GPA33 The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily, which contains. GPA33 may play a role in cell-cell recognition and signaling.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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