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Your Position: Home > Immune Checkpoint Proteins > OX40 Ligand > Recombinant Human OX40 Ligand / TNFSF4 / CD252 Protein

Human OX40 Ligand / TNFSF4 Protein (active trimer) (HPLC-verified)

  • Synonym
    OX40L,TNFSF4,CD252,Glycoprotein Gp34,TXGP1
  • Source
    Human OX40 Ligand (active trimer) (HPLC-verified) (OXL-H52Q8) is expressed from human 293 cells (HEK293). It contains AA Gln 51 -  Leu 183 (Accession # NP_003317).
    Predicted N-terminus: His
  • Molecular Characterization
    OX40 Ligand(Gln 51 -  Leu 183) NP_003317

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 16.9 kDa. The protein migrates as 25-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

Human OX40 Ligand (active trimer) (HPLC-verified) (Cat. No. OXL-H52Q8) SDS-PAGE gel

Human OX40 Ligand (active trimer) (HPLC-verified) on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Human OX40 Ligand (active trimer) (HPLC-verified) (Cat. No. ) HPLC images

A SEC-HPLC analysis showing over 90% of Human OX40 Ligand (active trimer) (HPLC-verified) (Cat. No. OXL-H52Q8)  present as active trimers with a molecular mass around 90 kDa.

Human OX40 Ligand (active trimer) (HPLC-verified)Human OX40 Ligand (active trimer) (HPLC-verified) (Cat. No. OXL-H52Q8) ELISA bioactivity

Immobilized Human OX40, Fc Tag (Cat. No. OX0-H5255) at 2 μg/mL (100 μL/well) can bind Human OX40 Ligand (active trimer) (HPLC-verified) (Cat. No. OXL-H52Q8) with a linear range of 2-31 ng/mL (QC tested).

FACS assay shows that recombinant Biotinylated FACS

FACS assay shows that Human OX40 Ligand (active trimer) (HPLC-verified) (Cat. No. OXL-H52Q8) can bind to 293T cell overexpressing human OX40. The concentration of OX40 Ligand used is 0.1 μg/ml (Routinely tested).

  • Background
    Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L), TAX transcriptionally-activated glycoprotein 1 and CD252, which belongs to the tumor necrosis factor family. TNFSF4 is the ligand for CD134 and is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation. The interaction of TNFSF4-TNFSF4 is involved in the pathogenesis of multiple autoimmune and inflammatory diseases such as systemic lupus erythematosus (SLE), carotid artery disease and cancer. Furthermore, similar to other TNF superfamily members, membrane-bound OX40 Ligand (TNFSF4) exists as a homotrimer. Human TNFSF4 shares 46% amino acid sequence identity with its mouse counterpart.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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