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Your Position: Home > Immune Checkpoint Proteins > DNAM-1 > DN1-H52H6

Human DNAM-1 / CD226 Protein, His Tag

  • Synonym
  • Source
    Human DNAM-1, His Tag (DN1-H52H6) is expressed from human 293 cells (HEK293). It contains AA Glu 19 - Asn 247 (Accession # NP_006557).
    Predicted N-terminus: Glu 19
  • Molecular Characterization
    Online(Glu 19 - Asn 247) NP_006557

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 27.9 kDa. The protein migrates as 36-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
Human DNAM-1, His Tag (Cat. No. DN1-H52H6) SDS-PAGE gel

Human DNAM-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Human DNAM-1, His TagHuman DNAM-1, His Tag (Cat. No. DN1-H52H6) ELISA bioactivity

Immobilized Human DNAM-1, His Tag (Cat. No. DN1-H52H6) at 2 μg/mL (100 μL/well) can bind Human CD155, Fc Tag (Cat. No. CD5-H5251) with a linear range of 3-51 ng/mL (QC tested).


Loaded Human CD155, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD5-H5254) on Protein A Biosensor, can bind Human DNAM-1, His Tag (Cat. No. DN1-H52H6) with an affinity constant of 0.57 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

FACS assay shows that recombinant Biotinylated FACS

FACS assay shows that Human DNAM-1, His Tag (Cat. No. DN1-H52H6) can bind to 293T cell overexpressing human CD155. The concentration of DNAM-1 used is 3 μg/mL (Routinely tested).

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