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Your Position: Home > Immune Checkpoint Proteins > DNAM-1 > Human DNAM-1 /CD226 Protein,C-Fc Tag

Human DNAM-1 / CD226 Protein, Fc Tag

  • Synonym
    DNAM1,CD226,PTA1
  • Source
    Human DNAM-1, Fc Tag (DN1-H5257) is expressed from human 293 cells (HEK293). It contains AA Glu 19 - Asn 247 (Accession # NP_006557).
    Predicted N-terminus: Glu 19
  • Molecular Characterization
    Unconjugated(Glu 19 - Asn 247) NP_006557

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 52.6 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human DNAM-1, Fc Tag (Cat. No. DN1-H5257) SDS-PAGE gel

Human DNAM-1, Fc Tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human DNAM-1, Fc TagHuman DNAM-1, Fc Tag (Cat. No. DN1-H5257) ELISA bioactivity

Immobilized Rhesus macaque CD155 (Cat. No. CD5-R52H5) at 5 μg/mL (100 μL/well) can bind Human DNAM-1, Fc Tag (Cat. No. DN1-H5257) with a linear range of 2-31 ng/mL (QC tested).

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

FACS assay shows that recombinant  Human DNAM-1 Protein, Fc Tag (Cat. No. DN1-H5257) can bind to 293T cell overexpressing human CD155. The concentration of DNAM-1 used is 0.03 μg/ml (Routinely tested).

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