This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 52.6 kDa. The protein migrates as 66-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human DNAM-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Loaded Human DNAM-1, Fc Tag (Cat. No. DN1-H5257) on Protein A Biosensor, can bind Human CD155, His Tag (Cat. No. CD5-H5223) with an affinity constant of 1.1 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Human DNAM-1, Fc Tag (Cat. No. DN1-H5257) on Protein A Biosensor, can bind Human Nectin-2, His Tag (Cat. No. PV2-H52E2) with an affinity constant of 0.66 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
FACS assay shows that recombinant Human DNAM-1 Protein, Fc Tag (Cat. No. DN1-H5257) can bind to 293T cell overexpressing human CD155. The concentration of DNAM-1 used is 0.03 μg/ml (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $250.00
Price(USD) : $1500.00
high quality SARS-CoV-2 S protein, S1 protein and human ACE2 protein based on the human cell expression platform of HEK293.
The affinity constant of SARS-CoV-2 S protein (active trimer) binding to human ACE2 protein was 35.3 nM as verified by SPR.
The affinity constant of human ACE2 protein binding to SARS-CoV-2 S1 protein was 16.7 nM as verified by SPR. Human ACE2 proteins was verified by SEC-MALS and bioactivity was verified by SPR, BLI and ELISA. Protocols are offered for free.
Binding signal of biotinylated human ACE2 and SARS-CoV-2 S protein RBD was inhibited by SARS-CoV-2 inhibitor with the IC50 10.69 nM.
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