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Mouse FCGRT&B2M Heterodimer Protein, His Tag&Twin-Strep Tag, produced by co-expression of FCGRT and B2M, has a calculated MW of 32.9 kDa (FCGRT) and 14.7 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with Twin-Strep tag at the C-terminus. The reducing (R) protein migrates as 42-48 kDa (FCGRT) and 15-17 kDa (B2M) respectively due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Mouse FCGRT&B2M Heterodimer Protein, His Tag&Twin-Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Mouse FCGRT&B2M Heterodimer Protein, His Tag&Twin-Strep Tag (Cat. No. FCM-M52W2) is more than 90% and the molecular weight of this protein is around 49-67 kDa verified by SEC-MALS.
Immobilized Ipilumimab at 5 μg/mL (100 μL/well) can bind Mouse FCGRT&B2M Heterodimer Protein, His Tag&Twin-Strep Tag (Cat. No. FCM-M52W2) with a linear range of 0.625-5 μg/mL (Routinely tested).
Mouse FCGRT&B2M Heterodimer Protein, His Tag&Twin-Strep Tag (Cat. No. FCM-M52W2) captured on NTA-Series S sensor chip can bind Herceptin with an affinity constant of 4.61 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Loaded Mouse FCGRT&B2M Heterodimer Protein, His Tag&Twin-Strep Tag (Cat. No. FCM-M52W2) on NTA Biosensor, can bind Herceptin with an affinity constant of 0.858 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Loaded Mouse FCGRT&B2M Heterodimer Protein, His Tag&Twin-Strep Tag (Cat. No. FCM-M52W2) on NTA Biosensor, can bind Mouse IgG Fc, Tag Free (Cat. No. IG1-M5208) with an affinity constant of 20 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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