This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 16.9 kDa. The protein migrates as 33-40 kDa under reducing (R) condition, and 55-66 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Mouse CD28, His Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Mouse CD28, His Tag (Cat. No. CD8-M52H6) is more than 90% and the molecular weight of this protein is around 58-75kDa verified by SEC-MALS.
Immobilized Mouse CD28, His Tag (Cat. No. CD8-M52H6) at 5 μg/mL (100 μL/well) on THE™ His Tag Antibody (Mouse) (0.1 μg/well) plate can bind Mouse B7-1, Fc Tag (Cat. No. CD0-M5259) with a linear range of 0.04-1 μg/ml (QC tested).
Mouse B7-1, Fc Tag (Cat. No. CD0-M5259) captured on CM5 chip via anti-human IgG Fc antibody can bind Mouse CD28, His Tag (Cat. No. CD8-M52H6) with an affinity constant of 49.6 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Loaded Mouse B7-1, Fc Tag (Cat. No. CD0-M5259) on Protein A Biosensor, can bind Mouse CD28, His Tag (Cat. No. CD8-M52H6) with an affinity constant of 4.7 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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