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Your Position: Home > Protein > FGF basic > BFF-H4117

Human FGF basic Protein, premium grade

  • Synonym
    FGF2,BFGF,FGFB,FGF basic,HBGF-2
  • Source
    Human FGF basic, premium grade(BFF-H4117) is expressed from E. coli cells. It contains AA Pro 143 - Ser 288 (Accession # P09038-4 ).
    Predicted N-terminus: Met
    It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
  • Molecular Characterization
    FGF basic Structure

    This protein carries no "tag"

    The protein has a calculated MW of 16.5 kDa. The protein migrates as 17 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Host Cell Protein
    <0.5 ng/μg of protein tested by ELISA.
  • Host Cell DNA
    <0.02 ng/μg of protein tested by qPCR.
  • Sterility
    The sterility testing was performed by membrane filtration method.
  • Mycoplasma
    Negative.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
FGF basic SDS-PAGE

Human FGF basic, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
FGF basic MALS images

The purity of Human FGF basic, premium grade (Cat. No. BFF-H4117) is more than 85% and the molecular weight of this protein is around 16-24 kDa verified by SEC-MALS.

Bioactivity-Bioactivity CELL BASE
 FGF basic CELL

Human FGF basic, premium grade (Cat. No. BFF-H4117) stimulates proliferation of HUVEC in the range of 0-20 ng/mL. The EC50 for this effect is 0.416-0.630 ng/mL (Routinely tested).

Bioactivity-ELISA
 FGF basic ELISA

Immobilized Human FGF basic, premium grade (Cat. No. BFF-H4117) at 2 μg/mL (100 μL/well) can bind Human Glypican 3, Fc Tag, low endotoxin (Cat. No. GP3-H5258) with a linear range of 4-125 ng/mL (QC tested).

 FGF basic ELISA

Immobilized Human FGF basic, premium grade (Cat. No. BFF-H4117) at 1 μg/mL (100 μL/well) can bind Human Glypican 1, Fc Tag (Cat. No. GP1-H5254) with a linear range of 1-39 ng/mL (Routinely tested).

Bioactivity-SPR
 FGF basic SPR

Immobilized Human FGF basic, premium grade (Cat. No. BFF-H4117) on CM5 Chip can bind Heparin with an affinity constant of 8.37 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
 FGF basic BLI

Loaded Biotinylated Heparin on SA Biosensor, can bind Human FGF basic, premium grade (Cat. No. BFF-H4117) with an affinity constant of 6.27 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    FGF basic (also known as FGF2 and HBGF-2) is an 18-34 kDa, heparin-binding member of the FGF superfamily of molecules (1-3). Superfamily members are characterized by the presence of a centrally placed beta -trefoil structure. FGF acidic (FGF-1) and FGF basic (FGF2) were the first two identified FGFs, and the designations acidic and basic refer to their relative isoelectric points. Human FGF basic is 288 amino acids (aa) in length. There are multiple start sites, four of which utilize atypical CUG codons, and one that initiates at an AUG start site (4 - 6). The four CUG start sites generate high molecular weight (HMW) FGF basic. There is a 34 kDa, 288 aa form, a 24 kDa, 210 aa form, a 22.5 kDa, 201 aa form, and a 22 kDa, 196 aa form. All are retained intracellularly, undergo extensive methylation, and possess one or more nuclear localization signals (NLS) (7-9). The AUG initiating form is 18 kDa and 155 aa in length. There is no signal sequence (ss). It is, however, secreted directly through the plasma membrane via a mechanism that appears to be dependent upon tertiary structure (10). In place of a ss, there is purportedly a 9 aa N-terminal prosegment that precedes a 146 aa mature segment (11). Early isolations of 18 kDa bovine FGF basic yielded 146 aa molecules, an effect attributed to the presence of acid proteases (12). The molecule contains a heparin-binding site (aa residues 128-144), and undergoes phosphorylation at Ser117 (13). There is also an ill-defined C-terminal NLS that may be more “functional” (or 3-dimensional) than structural (7). Human 146 aa FGF basic is 97% aa identical to mouse FGF basic (14).
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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