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This protein carries no "tag"
The protein has a calculated MW of 16.5 kDa. The protein migrates as 17 kDa under reducing (R) condition (SDS-PAGE).
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human FGF basic, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human FGF basic, premium grade (Cat. No. BFF-H4117) is more than 85% and the molecular weight of this protein is around 16-24 kDa verified by SEC-MALS.
Human FGF basic, premium grade (Cat. No. BFF-H4117) stimulates proliferation of HUVEC in the range of 0-20 ng/mL. The EC50 for this effect is 0.416-0.630 ng/mL (Routinely tested).
Immobilized Human FGF basic, premium grade (Cat. No. BFF-H4117) at 2 μg/mL (100 μL/well) can bind Human Glypican 3, Fc Tag, low endotoxin (Cat. No. GP3-H5258) with a linear range of 4-125 ng/mL (QC tested).
Immobilized Human FGF basic, premium grade (Cat. No. BFF-H4117) at 1 μg/mL (100 μL/well) can bind Human Glypican 1, Fc Tag (Cat. No. GP1-H5254) with a linear range of 1-39 ng/mL (Routinely tested).
Immobilized Human FGF basic, premium grade (Cat. No. BFF-H4117) on CM5 Chip can bind Heparin with an affinity constant of 8.37 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Loaded Biotinylated Heparin on SA Biosensor, can bind Human FGF basic, premium grade (Cat. No. BFF-H4117) with an affinity constant of 6.27 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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