Human PSME3 / PA28g Protein, His Tag

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Cat. No. / Size
Price
Qty
PS3-H5142-100ug
$185.00
PS3-H5142-1mg (200ug X 5)
$1110.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    PSME3, PA28gamma, REG-gamma

  • Source

    Human PSME3, His Tag (PS3-H5142) is expressed from E. coli cells. It contains AA Ala 2 - Tyr 254 (Accession # P61289-1).

    Predicted N-terminus: Met

    Request for sequence
  • Molecular Characterization

    PSME3 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 30.3 kDa. The protein migrates as 28-33 kDa under reducing (R) condition (SDS-PAGE).

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    PSME3 SDS-PAGE

    Human PSME3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

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Background

Proteasome activator complex subunit 3 (PSME3),a member of the PA28 family, is also known as 11S regulator complex subunit gamma (REG-gamma), activator of multicatalytic protease subunit 3 and proteasome activator 28 subunit gamma (PA28gamma). PSME3 activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. PSME3 can also facilitate the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. Furthermore,PSME3 may also be involved in cell cycle regulation.

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