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Your Position: Home > Immune Checkpoint Proteins > PD-1 > Rabbit PD-1 / PDCD1 Protein, Fc Tag

Rabbit PD-1 / PDCD1 Protein, Fc Tag

  • Synonym
    PDCD1,PD1,CD279,SLEB2
  • Source
    Rabbit PD-1, Fc Tag (PD1-RB5251) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # G1SUF0).
    Predicted N-terminus: Leu 25
  • Molecular Characterization
    PD-1(Leu 25 - Gln 167) G1SUF0

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 42.6 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Rabbit PD-1, Fc Tag (Cat. No. PD1-RB5251) SDS-PAGE gel

Rabbit PD-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Rabbit PD-1, Fc TagRabbit PD-1, Fc Tag (Cat. No. PD1-RB5251) ELISA bioactivity

Immobilized Mouse PD-L1, His Tag (Cat. No. PD1-M5220) at 10 μg/mL (100 μL/well) can bind Rabbit PD-1, Fc Tag (Cat. No. PD1-RB525) with a linear range of 3-50 ng/mL (QC tested).

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