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Your Position: Home > Immune Checkpoint Proteins > OX40 > Biotinylated Human OX40 / TNFRSF4 / CD134 Protein, Fc Tag

Biotinylated Human OX40, Fc Tag, Avi Tag (Avitag™)

  • Synonym
    TNFRSF4,OX40,CD134,OX40L receptor,ACT35,TXGP1L
  • Source
    MABSol® Biotinylated Human OX40, Fc Tag, Avi Tag (OX0-H82F7) is expressed from human HEK293 cells. It contains AA Leu 29 - Ala 216 (Accession # NP_003318).
    Predicted N-terminus: Leu 29
  • Molecular Characterization
    OX40(Leu 29 - Ala 216) NP_003318

    This protein carries a human IgG1 Fc fragment at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 48.6 kDa. The protein migrates as 66 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human OX40, Fc Tag, Avi Tag (Cat. No. OX0-H82F7) SDS-PAGE gel

Biotinylated Human OX40, Fc Tag, Avi Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated Human OX40, Fc Tag, Avi TagBiotinylated Human OX40, Fc Tag, Avi Tag (Cat. No. OX0-H82F7) ELISA bioactivity

Immobilized Human OX40 Ligand, Fc Tag (Cat. No. OXL-H526x) at 5 μg/mL (100 μl/well) can bind Biotinylated Human OX40, Fc Tag, Avi Tag (Cat. No. OX0-H82F7) with a linear range of 0.01-0.3 μg/mL (QC tested).

FACS assay shows that recombinant Biotinylated FACS

FACS assay shows that recombinant Biotinylated Human OX40, Fc Tag, Avi Tag (Cat. No. OX0-H82F7) can bind to 293T cell overexpressing human OX40L. The concentration of OX40 is 0.1 μg/ml (Routinely tested).

  • Background
    Tumor necrosis factor receptor superfamily member 4 (TNFRSF4) is also known as ACT35 antigen, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor, CD antigen CD134, OX40. OX40 / TNFRSF4 contains four TNFR-Cys repeats. TNFRSF4 is receptor for TNFSF4 / OX40L / GP34 and can interacts with TRAF2, TRAF3 and TRAF5.
  • References
  • Limited Use License

    The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

    Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1380.00

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