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Your Position: Home > Immune Checkpoint Proteins > CTLA-4 > CT4-M52H5

Mouse CTLA-4 / CD152 Protein, His Tag

  • Synonym
    CTLA4,CD152
  • Source
    Mouse CTLA-4, His Tag (CT4-M52H5) is expressed from human 293 cells (HEK293). It contains AA Glu 36 - Phe 162 (Accession # NP_033973).
    Predicted N-terminus: Glu 36
  • Molecular Characterization
    Online(Glu 36 - Phe 162) NP_033973

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 15.9 kDa. The protein migrates as 25-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Mouse CTLA-4, His Tag (Cat. No. CT4-M52H5) SDS-PAGE gel

Mouse CTLA-4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Mouse CTLA-4, His TagMouse CTLA-4, His Tag (Cat. No. CT4-M52H5) ELISA bioactivity

Immobilized Mouse B7-1, Fc Tag (Cat. No. CD0-M5259) at 2 μg/mL (100 µl/well),can bind Mouse CTLA-4, His Tag (Cat. No. CT4-M52H5) with a linear range of 0.02-0.8 ng/mL (QC tested).

Mouse CTLA-4, His TagMouse CTLA-4, His Tag (Cat. No. CT4-M52H5) ELISA bioactivity

Immobilized Mouse B7-2, Fc Tag (Cat. No. CD6-M5251) at 5 μg/mL (100 μL/well) can bind Mouse CTLA-4, His Tag (Cat. No. CT4-M52H5) with a linear range of 0.4-13 ng/mL (Routinely tested).

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