This protein carries a polyhistidine tag at the N-terminus
The protein has a calculated MW of 16.8 kDa. The protein migrates as 23 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human IL-17F (H161R), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human IL-17F (H161R), His Tag (Cat. No. ILF-H4240) at 10 μg/mL (100 μL/well) can bind Biotinylated Human IL-17 RA, Fc,Avitag (Cat. No. ILA-H82F1) with a linear range of 0.02-0.313 μg/mL (QC tested).
Immobilized Human IL-17F (H161R), His Tag (Cat. No. ILF-H4240) at 10 μg/mL (100 μL/well) can bind Human IL-17 RA, Fc Tag (Cat. No. ILA-H5257) with a linear range of 0.078-1.25 μg/mL (Routinely tested).
Immobilized Human IL-17F (H161R), His Tag (Cat. No. ILF-H4240) at 2 μg/mL (100 μL/well) can bind Recombinant Human IL-17RC Isoform 1 Fc Chimera with a linear range of 0.2-16 ng/mL (Routinely tested).
Loaded Human IL-17 RA, Fc Tag (Cat. No. ILA-H5257) on Protein A Biosensor, can bind Human IL-17F (H161R), His Tag (Cat. No. ILF-H4240) with an affinity constant of 299 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Human IL17RA & IL17RC Protein, Fc Tag&Fc Tag (Cat. No. ILC-H5257) on Protein A Biosensor, can bind Human IL-17F (H161R), His Tag (Cat. No. ILF-H4240) with an affinity constant of 1.79 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Human IL-17F (H161R), His Tag (Cat. No. ILF-H4240) on NTA Biosensor, can bind Human IL-17 RC, Fc Tag (Cat. No. ILC-H5259) with an affinity constant of 288 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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