Human IgG1 (N297A) Kappa Isotype Control, premium grade (mAb, carrier free)

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Cat. No. / Size
Price
Qty
DNP-MB273-100ug
$190.00
DNP-MB273-1mg (250ug X 4)
$830.00
DNP-MB273-25mg
$1855.00
DNP-MB273-100mg
$6180.00
ETA of in-stock products:2 business days

Product Details

  • Source

    Human IgG1 (N297A) Kappa Isotype Control, premium grade (mAb) is a chimeric monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which combines the variable region of a mouse monoclonal antibody with human IgG1 constant domain.

  • Antibody Type

    Recombinant Monoclonal

  • Isotype

    Human IgG1 (N297A), Kappa

  • Host Species

    Human

  • Specificity

    This product is a specific antibody specifically reacts with DNP.

  • Application

    Application
    Recommended Usage
    ELISA
    1:8000-1:80000
  • Purification

    Protein A purified / Protein G purified.

  • Aggregation

    Less than 5%, as determined by SEC-MALS.

  • Concentration

    Please refer to the Certificate of Analysis (CoA).

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

    25 mg or larger size will be supplied as liquid and shipped by dry ice. Please inquire the dry ice shipping cost.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-ELISA

     IgG control ELISA

    Immobilized DNP-Albumin Conjugate, Bovine at 10 μg/mL (100 μL/well) can bind Human IgG1 (N297A) Kappa Isotype Control, premium grade (mAb) (Cat. No. DNP-MB273) with a linear range of 0.1-1 ng/mL (QC tested).

    Protocol

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Background

A hapten is a small molecule that can elicit an immune response only when conjugated with a large carrier such as a protein. Typical haptens include drugs, urushiol, quinone, steroids, etc. Peptides and non-protein antigens usually need conjugating to a carrier protein (such as BSA (bovine serum albumin) or KLH (keyhole limpet hemocyanin) to become good immunogens). Additionally, haptens should be administered with an adjuvant to ensure a high quality immune response. It is important that the hapten design (preserving greatly the chemical structure and spatial conformation of target compound), selection of the appropriate carrier protein and the conjugation method are key conditions for the desired specificity anti-hapten antibodies. We design anti-hapten antibodies based on the HaptenDB information.

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