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Your Position: Home > Immune Checkpoint Proteins > CTLA-4 > Mouse CTLA-4 / CD152 Protein, Fc Tag

Mouse CTLA-4 / CD152 Protein, Fc Tag

  • Synonym
    CTLA4,CD152
  • Source
    Mouse CTLA-4, Fc Tag (CT4-M5256) is expressed from human 293 cells (HEK293). It contains AA Glu 36 - Phe 162 (Accession # NP_033973).
    Predicted N-terminus: Glu 36
  • Molecular Characterization
    Online(Glu 36 - Phe 162) NP_033973

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 40.6 kDa. The protein migrates as 55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mouse CTLA-4, Fc Tag (Cat. No. CT4-M5256) SDS-PAGE gel

Mouse CTLA-4, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Mouse CTLA-4, Fc TagMouse CTLA-4, Fc Tag (Cat. No. CT4-M5256) ELISA bioactivity

Immobilized Mouse B7-1, His Tag (Cat. No. CD0-M5228) at 2μg/mL (100 μL/well) can bind Mouse CTLA-4, Fc Tag (Cat. No. CT4-M5256) with a linear range of 0.12-0.5 ng/mL (QC tested).

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