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Your Position: Home > Immune Checkpoint Proteins > B7-H5 > Human B7-H5 / Gi24 / VISTA Protein, Fc Tag

Human B7-H5 / Gi24 / VISTA Protein, Fc Tag

  • Synonym
    B7-H5,SISP1,Gi24,VISTA
  • Source
    Human B7-H5, Fc Tag (B75-H5259) is expressed from human 293 cells (HEK293). It contains AA Phe 33 - Ala 194 (Accession # AAH20568).
    Predicted N-terminus: Phe 33
  • Molecular Characterization
    Online(Phe 33 - Ala 194) AAH20568

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 44.8 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human B7-H5, Fc Tag (Cat. No. B75-H5259) SDS-PAGE gel

Human B7-H5, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

  • Background
    Platelet receptor Gi24, also known as B7-H5 and stress-induced secreted protein-1 (Sisp-1), is a protein that in humans is encoded by the C10orf54 gene, which contains 1 Ig-like (immunoglobulin-like) domain. As for C10orf54 gene, C10orf54 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state on ESCs. Human C10orf54 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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